Web3 mrt. 2024 · We report that BLs overexpress SBP enzymes, phosphoglycerate dehydrogenase (PHGDH) and phosphoserine aminotransferase 1 (PSAT1). Both genes are controlled by the MYC-dependent ATF4 transcription factor. Genetic ablation of PHGDH/PSAT1 or chemical PHGDH inhibition with NCT-503 decreased BL cell lines … WebMechanistically, we identified ATF4 as a novel regulator which coordinates with N-Myc to directly activate ASCT2 expression. Of note, ASCT2 expression, which correlates with …
Reactivation of Myc transcription in the mouse heart unlocks its ...
Web18 dec. 2024 · ATF4 is an important protective gene that allows cells to adapt to the aforementioned stress factors. It has been previously reported that, compared with normal tissue, primary tumors in human tissue have … Web5 jan. 2024 · MYC works in concert with ATF4 to elevate the expression of amino acid transporters, which stimulate the uptake of essential amino acids (EAAs) into the cell, ultimately supporting sustained mTORC1 signaling. coronation street psychic
Serine Biosynthesis Pathway Supports MYC-miR-494-EZH2 Feed …
Web5 aug. 2024 · We transduced MYC-ER HMECs with the CRISPR library in biological duplicate, selected for transduced cells with puromycin, treated half of the cells with tamoxifen (TAM) to induce MYC activity, and isolated genomic DNA (gDNA) 8 and 16 days after puromycin removal (Figure 1 A).The representation of randomly integrated sgRNAs … WebBL cells were transduced with siRNA targeting ATF4, incubated for 48 h, then the expression of ATF4, PHGDH, PSAT1, and MYC genes was analyzed with RQ-PCR. In A and B, ... WebNational Center for Biotechnology Information coronation street public school