WebSep 6, 2016 · An additional copy of fkbO, fkbL, fkbP, fkbD, or fkbM was individually introduced into S. tsukubaensis D852 under the control of ermE* promoter to overexpress each of these five genes. FK506 production by these mutant strains increased by 13–39 % compared to the wild-type strain. WebJan 9, 2014 · The chorismatase FkbO is a chorismate-converting enzyme from a Streptomyces species. The crystal structure of FkbO with a competitive inhibitor was …
5A3K - RCSB
WebDec 10, 2014 · The presented procedure starts with an optimised method for purifying chorismate from Escherichia coli culture supernatants, which is followed by conversion into isochorismate with the isochorismate synthase EntC, removal of the remaining chorismate by FkbO and a final purification step using an automated flash chromatography system. Web1 mM chorismate was dissolved in Tris buffer (100 mM, pH 7.0) containing 18 O-labeled water (97%) and incubated with FkbO or Hyg5 (100 µg/mL) at RT for 2 h. To avoid 18 O … snapshot testing using jest
NMR spectra of metabolites produced by the action of …
WebMar 2, 2013 · ENZYME entry: EC. 3.3.2.13. chorismate + H2O <=> (3R,4R)-3,4-dihydroxy-3,4-dihydrobenzoate + pyruvate. The enzyme found in several bacterial species is involved in the biosynthesis of macrocyclic polyketides. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc. WebDec 5, 1997 · A mechanism for FkbO is proposed based on both interactions between the inhibitor and the surrounding amino acids and an FkbO structure with chorismate modelled in the active site. We suggest that the methylene group of the chorismate enol ether takes up a proton from an active-site glutamic acid residue, thereby initiating chorismate … WebDownload scientific diagram The role of FkbO and Hyg5 in polyketide starter unit production from chorismate. from publication: Biosynthesis of the immunosuppressants … snapshot testing in react native