WebTo select an inactivation agent for the preparation of an effective IHNV vaccine, rainbow trout were immunized with mineral oil emulsions of IHNV vaccines inactivated by formaldehyde, binary ethylenimine (BEI), or β-propiolactone (BPL). ... binary ethylenimine (BEI), or β-propiolactone (BPL). The fish were challenged 8 weeks after … WebThe preparation and application of the aziridine compound binary ethylenimine (BEI) and the necessary conditions for and controls of the inactivation process are described …
Validation of the inactivant binary ethylenimine for inactivating ...
WebAug 31, 2024 · Different methods of virus inactivation are well known in the art and include, without limitation, formalin, binary ethylenimine (BEI) and beta-propiolactone. [0054] If the vaccine comprises a killed virus, it may optionally be adjuvanted. WebWhen binary ethylenimine was prepared directly in the virus suspension only 2-bromoethylamine hydrobromide gave acceptable inactivation rates. The reduced … cowshed pattingham menu
Genome shuffling and high-throughput screening of
Binary ethylenimine (BEI) is a preparation of aziridine. It can be produced by heating bromoethylamine hydrobromide or 2-aminoethyl hydrogen sulfate in the presence of sodium hydroxide (Gabriel method). It contains at least one three-membered ring which is very reactive because of the ring strain. It is used to … See more BEI is very hazardous since it attacks nucleic acids and proteins as described above. It can be neutralised by sodium thiosulfate; the thiosulfate is a nucleophile which opens the three-membered ring. The presence of … See more • Bahnemann, H. G. (1976). "Inactivation of viruses in serum with binary ethyleneimine". Journal of Clinical Microbiology. 3 (2): 209–210. ISSN 0095-1137 See more WebAug 1, 1990 · The preparation and application of the aziridine compound binary ethylenimine (BEI) and the necessary conditions for and controls of the inactivation process are described and discussed. A computer program has been written for assistance in the use of BEI for controlled inactivation of viral antigens. WebOct 1, 2005 · BEI was gradually adopted as the principal inactivation agent for FMDV vaccine preparation due to its stability, superior inactivation kinetics, reduced residual … cowshed oxfordshire